Gst gene fusion manual

This chapter describes the use of glutathione S-transferase (GST) gene fusion proteins as a method for inducible, high-level protein expression and purification from bacterial cell lysates. The protein is expressed in a pGEX vector, with the GST moiety located at . The Glutathione S-transferase (GST) Gene Fusion System is an integrated system for the expression, purification and detection of fusion proteins produced in E. coli. The system consists of three major components: pGEX plasmid vectors*, two GST Purification Modules and the GST Detection Module. A series of site-specific proteases complements the www.doorway.ru Size: KB. GST-fusion proteins are typically eluted from glutathione agarose with excess glutathione. Alternatively, the GST-fusion expression vector can be engineered to encode a recognition site for a specific protease, such as thrombin or factor Xa, between the GST structural gene and the gene of interest. Once the fusion protein is bound to the.

Bacterial vector for expressing GST fusion proteins with a PreScission protease site. For other reading frames, use pGEX-6P-2 or pGEX-6P The Glutathione S-transferase (GST) Gene Fusion System is an integrated system for the expression, purification and detection of fusion proteins produced in E. coli. The system consists of three major components: pGEX plasmid vectors*, two GST Purification Modules and the GST Detection Module. A series of site-specific proteases complements the. In-Fusion® HD Cloning Kit User Manual () www.doorway.ru Takara Bio USA, Inc. Page 5 of 15 II. List of Components All In-Fusion HD Cloning kits contain 5X In-Fusion HD Enzyme Premix, linearized pUC19 Control Vector (50 ng/μl), and 2 kb Control Insert (40 ng/μl). Store all components at °C.

The Glutathione S-transferase (GST) Gene Fusion System is an integrated system for the expression, purification and detection of fusion proteins produced in E. coli. The system consists of three major components: pGEX plasmid vectors*, two GST Purification Modules and the GST Detection Module. A series of site-specific proteases complements the. In-Fusion® HD Cloning Kit User Manual () www.doorway.ru Takara Bio USA, Inc. Page 5 of 15 II. List of Components All In-Fusion HD Cloning kits contain 5X In-Fusion HD Enzyme Premix, linearized pUC19 Control Vector (50 ng/μl), and 2 kb Control Insert (40 ng/μl). Store all components at –20°C. GST-fusion protein purification. Glutathione is a tripeptide (Glu-Cys-Gly) that is the specific substrate for glutathione S-transferase (GST). When reduced glutathione is immobilized through its sulfhydryl group to a solid support, such as cross-linked beaded agarose, it can be used to capture pure GST or GST-tagged proteins via the enzyme-substrate binding reaction.